| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Nashua South | A | Carlo | Hulfacher | Compoh | Miranda | 47.37 | |
| 2 | Souhegan | A | Steele | Hamele | Pariselli | Dickinson | 47.07 | BG Mini Meet @ Souhegan |
| 3 | Milford | A | Hardwick | Constable | Naun | Kimball | 44.92 | home meet 3 |
| 4 | Windham | A | Cerami | Bomba | Neal | Manti | DNS | Portsmouth |
| 5 | Nashua North | A | Dunning | Harris-Lopez | Provost | Smith | 43.94 | |
| 6 | Bedford | A | McDonald | Russell | Manfield | Graves | 45.84 | Bedford vs Concord |
| 7 | Manchester Central | A | Dimande | Kelley | Jerome | Preston | 47.14 | Cenrral/Goffstown |
| 8 | Alvirne | A | Prescott | Fay | Flaherty | Santos-Veras | 48.00 |