| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Assumption |
C | Bicknell | Crockett | Strmiska | Prario | DNS | Throwers |
| 3 | A | Stroh | Gravell | Hallet | Allen | 50.38 | Northeast-10 Outdoor Track & Field Championships | |
| 4 | Merrimack |
A | DiPerri | Laubstein | Sullivan | Faivre | 49.81 | UMASS Invitational |
| 5 | Assumption |
A | Rippstein | Hirschfeld | Garcia-Baez | Steffon | 49.97 | Outdoor last year same girls |
| 6 | Assumption |
B | Offley | Digregorio | Moran | Strmiska | 51.12 | WSU |