| S | NHIAA State Records | 48.68 | N McMorrowe, A Arena, O Rogeau, V Williams | Milford | 2016 |
| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | |
|---|---|---|---|---|---|---|---|
| 1 | Nashua South | A | Ostrowski | Braswell | Cruz | Ross | 49.29 |
| 2 | Pinkerton | A | Wheaton | Sippel | Connors | Roberts | 49.46 |
| 3 | Merrimack | A | Best | Farrell | Ogbunamiri | Dillon | 49.65 |
| 4 | Exeter | A | Thompson | Poulin | Kunysz | Kunysz | 50.23 |
| 5 | Central | A | Demers | Barber | Bangasimbo | Masumbuko | 50.66 |
| 6 | Coe-Brown | A | Malloy | Cowan | Chavda | Lee | 50.95 |
| 7 | Windham | A | Parker | Murray | Draeger | Smith | 51.12 |
| 8 | Kennett | A | Tuttle | Habert-Jaques | Wheat | Fayle | 51.48 |
| 9 | Portsmouth | A | Nada | Arakelian | Bancroft | Jenkins | 51.50 |
| 10 | Concord | A | Quirk | Sawyer | Caceres | Mullen | 51.54 |
| 11 | Nashua North | A | Juris | Tavarez | Thomas | Di Vernieri | 51.59 |
| 12 | Alvirne | A | Hartson | Apitz | Coppi | Mckoy-Sophos | 51.75 |
| 13 | Milford | A | Nassy | Gelinas | Nassy | Sawyer | 52.06 |
| 14 | Newfound | A | McKellar | Paul | Glidden | MacDonald | 52.12 |
| 15 | Belmont | A | Watson | Edgren | McDonald | Winslow | 52.41 |
| 16 | Newmarket | A | Madore | Santiago | Hill | Bussell | 53.94 |